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1Arun Kumar Sharma, 1Sapna Kumari, 1Vinay Sharma*, 2Jyoti Saxena
1Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, India.
2Department of Biochemical Engineering, Bipin Tripathi Kumaon Institute of Technology, Dwarahat, Uttrakhand.
*Corresponding Author
Prof. Vinay Sharma
Department of Bioscience and Biotechnology,
Banasthali University, Rajasthan, India.
Abstract
Background: Because of the several applications of lipases in industry, there is a necessitate to investigate their uniqueness, because lipases isolated from diverse sources may have diverse features. Objective: The aim of this work was to screen the potent lipase producing soil fungi by submerged fermentation. Materials and methods: Fungal strains were isolated from 4 different soil samples and used for production of lipase in submerged fermentation (SmF). Results: Results were assessed both in terms of lipase activity and specific activity. Among all the tested fungal strains (21), highest production of lipase (185.38 ± 2.58 U/ml/min) was obtained from isolate S3St6, which was isolated from petrol pump soil sample. Conclusion: Based on quantitative screening of 21 fungal strains in SmF, isolate S3St6 was recognized as potent producer of lipase.
Keywords: Lipases, fungi, submerged fermentation, soil sample, quantitative screening
