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K. Saraswathi1 B. Mahalakshmi2, V. Rajesh3, P. Arumugam3*
1Karpaga Vinayaga College of Engineering and Technology, Madhuranthagam, Kancheepuram-603 308, Tamil Nadu, India
2Meenakshi College for Women, Kodambakkam, Chennai-600 024, Tamil Nadu, India
3ARMATS Biotek Training and Research Institute, Guindy, Chennai-600 032, Tamil Nadu, India
*Address for Corresponding author
Dr. P. Arumugam
Industrial Fermentation Technology Division, ARMATS Biotek Training and Research Institute, Chennai-600 032, Tamil Nadu, India
Abstract
Objective: Atalantia racemosa belongs to the family Rutaceae, comprises of 11 species which are closely-related. The present study was mainly done to evaluate and identify the phyto-compounds present in the leaves of Atalantia racemosa. Materials and Methods: Phytochemical analysis and Thin layer chromatography proved to be a potent method for the presence of phyto-constituents. Also, the GC-MS analysis was performed in order to find out the active compounds in the extract which might be responsible for antioxidant, antibacterial activity. Results: Phytochemical investigation revealed the presence of phenols, flavonoids, steroids, terpenoids, etc. Toluene: Ethyl acetate: Methanol was preferred as the solvent system for the separation of compounds by chromatographic technique. The study revealed that Atalantia racemosa explored seven to eight bioactive compounds such as Beta-Asarone, Cis-Lanceol, Catechol from GC-MS analysis. Conclusion: The leaves of Atalantia racemosa could be considered as a potent antioxidant source against many free radicals, antibacterial activity. Presence of phenolic compounds, flavonoids might be responsible for antioxidant activity. Tannin-rich compounds would be definitely responsible for antibacterial activity.
Keywords: Catechol, flavonoids, GC-MS, phenols, steroids, retention time, thin layer chromatography
