Research Articles

2023  |  Vol: 8(5)  |  Issue: 5(September-October) | https://doi.org/10.31024/apj.2023.8.5.4

Evaluation of Cytotoxic, Antibacterial and Antioxidant activities of leaf extract of Coleus ambionicus

Amisha Murugesan Angel, W. A. Manjusha*, Briscilla C.

Department of Biotechnology, Malankara Catholic College Mariagiri, Affiliated to Manonmaniam Sundaranar University, Tirunelveli, 627012, Tamil Nadu, India

*Address for Corresponding author

Dr. W. A. Manjusha

Head and Assistant Professor,

Department of Biotechnology, Malankara Catholic College Mariagiri, Tamil Nadu, India

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Abstract

Objective: Coleus ambionicus has therapeutic properties attributed to its natural bio active compounds which are highly valued in the pharmacological industry. The present study evaluated the antibacterial, antioxidant & anticancer activity of coleus ambionicus leaf extract. Material and methods: The organic solvents such as ethanol, methanol, ethyl acetate and chloroform were used for the extraction of bioactive compounds. The antibacterial activity was screened by disc diffusion method and antioxidant activity was determined by reducing power method. Results and conclusion: The ethanol extract showed high antibacterial activity against Klebsiella pneumonia and the same extract was showed highest antioxidant activity. So, the ethanol extract of Coleus amboinicus was subjected to anticancer activity against A549, the lung cancer cell line and it showed dose dependent inhibition of lung cancer cell line. For the identification of bioactive compounds, the ethanol extract was subjected to column chromatography and Gas Chromatography-Mass Spectroscopy (GC-MS) analysis. GC-MS analysis showed the presence of three bio active compounds viz Phenol 2-methyl-5-(1-methylethyl), 2,4-Di-tert-butylphenol and Bis(2-ethylhexyl) phthalate and these bioactive compounds are responsible for the pharmacological properties of Coleus amboinicus.

Keywords: Coleus amboinicus, pharmacological activity, bioactive compounds, GC-MS